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Journal of Food and Nutrition Research
Online First Articles

Drdolová, Z. – Belej, Ľ. – Golian, J. – Benešová, L. – Čurlej, J. – Zajác, P.
Comparison of specificity and sensitivity of biochip and real-time polymerase chain reaction for pork in food


Zuzana Drdolová, Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia. E-mail: xdrdolova@uniag.sk

Received 30 January 2019; 1st revised 24 April 2019; accepted 7 May 2019; published online 31 July 2019

Súhrn: Adulteration and food fraud are serious global problems. Analytical methods regarding animal species should represent a comprehensive tool for identification and possible quantification of the components. The aim of the study was to confirm usability and sensitivity of the MEAT 5.0 LCD-Array Kit (version 5.0, Chipron, Berlin, Germany) for forensic analysis of food, which simultaneously detects 24 kinds of animals. We verified its reliability for identification of pork mixed with meat of other 15 animal species. Mixtures of animal species meat were tested in the raw state and after heat treatment. For comparison, real-time polymerase chain reaction innuDETECT Pork Assay (Analytic Jena, Berlin, Germany) with quantitative potential was used. A standard pork DNA curve was established and the detection limit was determined. MEAT 5.0 LCD-Array Kit was found to have a high degree of robustness, probe specificity and repeatability, with a detection limit down to 0.5 % (w/w) but did not allow quantification. We also used both test methods for analysis of commercial products. By these analyses, we identified the presence of pork DNA in 14 products for which this fact was not reported on the product label.

Kľúčové slová: meat; pork; biochip; detection; real-time polymerase chain reaction

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