Scientific journal

Journal of Food and Nutrition Research
Online First Articles

Krivohlavek, A. – Žuntar, I. – Ivešić, M. – Mandić Andačić, I. – Šikić, S. – Vrebčević, M.
Sibutramine in slimming food supplements on the Croatian market determined by validated high-pressure liquid chromatography-electrospray tandem mass spectrometry method

Adela Krivohlavek, Department of Environmental Protection and Health Ecology, Andrija Stampar Teaching Institute of Public Health, Mirogojska 16, 10 000 Zagreb, Croatia. E-mail:; tel.: +385(0)14696241; fax: +385(0)14678015

Received 15 February 2016; 1st revised 16 May 2016; accepted 7 June 2016; published online 14 July 2016.

Summary: People are trying to solve their obesity problem with easy solutions like using herbal products. The most dangerous are those to which medicines, like sibutramine, are added, since patient believes that the supplement is mostly herbal-based and thus safe for use. Our study aimed at helping to control sibutramine in slimming food supplements on the Croatian market. Analysis of sibutramine in such products was done by using high-pressure liquid chromatography-electrospray tandem mass spectrometry. For sibutramine, and N,N-didesmethylsibutramine as an internal standard, mass spectro­metry detection was performed in positive mode using Multiple Reaction Monitoring. The drugs were isolated using simple methanol extraction and were separated using reversed-phase liquid chromatography on a Zorbax SB C18 column (150 mm × 2.1 mm, particle size 3.5 µm; Agilent Technologies, Santa Clara, California, USA) with a gradient elution using 0.1% formic acid in acetonitrile and 0.1% formic acid in water as a mobile phase at a flow rate of 0.2 ml·min-1. The study showed that one-fifth of the samples tested over a period of six years contained sibutramine. Regular market control using the described method could prevent health risk to population.

Keywords: sibutramine; food supplements; liquid chromatography-electrospray tandem mass spectrometry

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