Scientific journal

46 2007

Journal of Food and Nutrition Research
Summary No. 3 / 2007

Detection of Shiga toxin-producing Escherichia coli in meat swabs by TaqMan real-time PCR targeting stx genes
Journal of Food and Nutrition Research, 46, 2007, No. 3, s. 97-100

Dobroslava Bujňáková, Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4/6, SK - 04001 Košice, Slovakia. Tel. +421 55 7287842, fax +421 55 7287842. E-mail:

Summary: Reliable methods for the detection and characterization of Shiga toxin-producing Escherichia coli (STEC) are essential for food safety. In this study, two pairs of PCR primers with respective TaqMan probes were used in real-time PCR for this purpose. The method efficiently amplified all variants of the Shiga toxin genes stx1 or stx2. A detection limit of 101 CFU per sample was determined for the complete method with meat swab samples artificially contaminated with reference E. coli strains positive for Stx1 or Stx2 production. The optimized complete detection system was suitable for rapid and sensitive detection of Shiga toxin-producing E. coli strains in meat swabs.

Keywords: Escherichia coli; real-time PCR; shiga toxin; enterohemorrhagic; meat swabs

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