Scientific journal

53 2014

Journal of Food and Nutrition Research
Summary No. 1 / 2014

Andrés, V. – Villanueva, M. J. – Mateos-Aparicio, I. – Tenorio, M. D.
Colour, bioactive compounds and antioxidant capacity of mixed beverages based on fruit juices with milk or soya
Journal of Food and Nutrition Research, 53, 2014, No. 1, s. 71-80

María-José Villanueva, Department of Food Science, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal s/n., 28040 Madrid, Spain. Tel.: +34 91 3941808, e-mail:

Summary: Fruit beverages are a source of phytochemicals, vitamins and minerals, with a high hydration capacity. The aim of this study was to evaluate mixed beverages based on fruit juices with milk or soya regarding their colour (L*, a*, b*), antioxidant compounds (carotenoids, ascorbic acid and polyphenols) and antioxidant activity. The b* parameter, which is associated with a yellow-orange colour, presented a positive correlation with beta-carotene concentration (r2 = 0.917). Beta-Carotene was the main carotenoid (58–3547 mg·l-1), followed by zeaxanthin (37–97 µg·l-1) and lutein (5–15 µg·l-1). The concentration of deliberately added ascorbic acid ranged between 78 mg·l-1 and 993 mg·l-1. The HPLC methods used proved to be suitable for the analysis of carotenoids and vitamin C in these mixed beverages. Polyphenols are naturally present in these mixed beverages (151–503 mg·l-1). The antioxidant capacity of each mixed beverage was assessed in vitro by the ferric reducing antioxidant power (FRAP) method and ranged from 65 mmol·l-1 to 376 mmol·l-1 (expressed as Trolox equivalents). The antioxidant capacity of these beverages was mainly due to the vitamin C (97.7%) and, in a minor proportion, due to polyphenols (2.3%). Discriminant analysis was applied to these samples of mixed beverages and samples from ‘tropical’ and ‘soyjuice’ groups were found to be the best classified into their commercial denominations.

Keywords: fruit juice; milk; soya; mixed beverage; colour; carotenoids; ascorbic acid; antioxidant activity

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