Food Research Institute

Kubincová, J. – Višňovský, J. – Rosenberg, M. – Hronská, H. – Chochulová, A. – Kubinec, R. – Blaško, J.
Application of high-temperature GC-MS for simultaneous identification and quantification of glycosidic forms of iridoids and flavonoids in plant samples
Journal of Food and Nutrition Research, 55, 2016, No. 4, s. 374-381

Jaroslav Blaško, Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská Dolina CH-2, Ilkovičova 6, 84215 Bratislava, Slovakia. E-mail: blasko@fns.uniba.sk, tel. +421 2 602 96 330

Received 8 September 2016; 1st revised 14 November 2016; accepted 16 November 2016; published online 22 November 2016

Summary: High-temperature gas chromatography with mass spectrometry detection (HTGC-MS) was applied to separation and identification of iridoids (aucubin, catalpol), flavonoid aglycone (quercetin, apigenin, luteolin) and flavonoid glycosides (quercetin 3-O-alpha-D-arabinoside, quercetin 3-O-beta-D-galactoside and quercetin 3-O-beta-D-glucoside) in standard mixtures and 26 real natural matrices. Conditions of HTGC-MS were set in accordance with the best achieved separation of analytes and the highest value of signal in the used system. The developed two-step derivatization process allowed direct silanization of polar analytes in the plant material without the need of extraction. For the tested analytes, the calculated limits of detection were in the range of 2–35 mg·kg^-1 and the values of retention indices ranged from 2?858 for aucubin to 3?859 for quercetin 3-O-alpha-D-arabinoside.

Keywords: iridoid; flavonoid; glycoside; high-temperature gas chromatography; mass spectrometry; plant

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