Food Research Institute

Machyňáková, A. – Hroboňová, K.
Simultaneous determination of coumarin derivatives in natural samples by ultra high performance liquid chromatography
Journal of Food and Nutrition Research, 56, 2017, No. 2, s. 179-188

Katarína Hroboňová, Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, Slovakia. E-mail: katarina.hrobonova@stuba.sk

Received 24 February 2017; 1st revised 31 March 2017; accepted 11 April 2017; published online 22 May 2017

Summary: Ultra high performance liquid chromatography method coupled with ultraviolet and fluorescence detection was developed for determination of coumarin derivatives and metabolites (esculin, daphnetin, fraxetin, umbelliferone, 4-methylumbelliferone, 4-hydroxycoumarin, scoparone, coumarin, herniarin). The separations were performed using octadecyl or phenyl-hexyl silica-based analytical columns (50 mm × 4.6 mm, 1.8 µm particle size) at gradient elution with mobile phase consisting of 0.3% aqueous acetic acid : acetonitrile (9 : 1) and acetonitrile (0–35 % acetonitrile over 3.5 min). The parameters of system suitability (repeatability of retention time and peak area) and validation (linearity range, limits of detection and quantification, precision and recovery) were determined for evaluation of the method. The limits of quantification were found in micrograms per millilitre range for ultraviolet detection and in nanograms per millilitre for fluorescence detection. Practical applicability of the method was demonstrated by analysing plant and propolis samples. Accuracy of the method was assessed at three concentration levels and values of the average percentage recoveries were higher than 88 % with relative standard deviation lower than 5 %.

Keywords: simple coumarins; natural samples; ultra high performance liquid chromatography; ultraviolet detection; fluorescence detection

Download:
  (pdf, 642.96 Kb, 4107x)