Scientific journal

56 2017

Journal of Food and Nutrition Research
Summary No. 3 / 2017

Hohnová, B. – Šalplachta, J. – Karásek, P.
Pressurized hot water extraction followed by high-performance liquid chromatography for determination of polyphenols in Sambucus nigra L. branches in dependence on vegetative period of the plant
Journal of Food and Nutrition Research, 56, 2017, No. 3, s. 299-303

Barbora Hohnová, Institute of Analytical Chemistry of the Czech Academy of Science, v. v. i., Veveří 967/97, 602 00 Brno, Czech Republic. Tel.: +420 532 290 172; fax: +420 541 212 113; e-mail:

Received 27 February 2017; 1st revised 19 April 2017; 2nd revised 31 May 2017; accepted 7 June 2017; published online 3 September 2017

Summary: Pressurized hot water extraction (PHWE) was optimized for the determination of selected polyphenols (catechin, epicatechin, rutin, quercetin and chlorogenic acid) in Sambucus nigra L. branches. Pressure 15 MPa, temperature 100 °C and extraction time 1 × 5 min were found to be sufficient for the extraction of target polyphenols from the matrix. Chlorogenic acid and rutin were the most abundant substances, the highest contents of them were observed in December (922 mg·kg-1 for rutin and 108 mg·kg-1 for quercetin).

Keywords: Sambucus nigra L. branches; pressurized hot water extraction; high performance liquid chromatography; polyphenols

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