Scientific journal

57 2018

Journal of Food and Nutrition Research
Summary No. 4 / 2018

Zdeňková, K. – Akhatova, D. – Fialová, E. – Krupa, O. – Kubica, L. – Lencová, S. – Demnerová, K.
Detection of meat adulteration: Use of efficient and routine-suited multiplex polymerase chain reaction-based methods for species authentication and quantification in meat products
Journal of Food and Nutrition Research, 57, 2018, No. 4, s. 351-362

Kamila Zdeňková, Department of Biochemistry and Microbiology, University of Chemistry and Technology, Technická 5, 166 28 Prague 6, Czech Republic. E-mail: kamila.zdenkova@vscht.cz, tel. +420220445196

Received 12 March 2018; 1st revised 18 April 2018; accepted 20 April 2018; published online 5 September 2018

Summary: The increase in the extent of meat adulteration is the reason for a need for an effective method for authentication of meat products. DNA-based polymerase chain reaction (PCR) is a well suited alternative for this purpose. Furthermore, the method facilitates quantification of animal DNA in meat products based on the correlation between target copy amounts and cycle numbers in quantitative PCR. We designed and experimentally verified PCR primer systems for identification of beef, pork, horse and poultry (chicken, turkey) meat. Mitochondrial and chromosomal markers were used. The mitochondrial cytochrome b gene was used as a marker for qualitative multiplex endpoint PCR and single-copy chromosomal genes (cyclic-GMP-phosphodiesterase gene for cattle, beta-actin gene for pig, interleukin-2 gene for chicken, myostatin gene for mammals and poultry) were used for multiplex quantitative PCR analyses. The reliability of both methods was confirmed by analysing of mixed samples prepared with or without heat treatment. The methods were then applied to 14 commercially available products typical for the Czech Republic, including sausages or salami. Discrepancies were observed between the DNA analysis and the meat content declared for the tested products, as two of the samples did not correspond to qualitative requirements and other four failed to meet quantitative requirements. The proposed PCR-based methodology was shown to be useful for the disclosure of meat adulteration.

Keywords: DNA; multiplex polymerase chain reaction; authentication; meat

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