Scientific journal
Journal of Food and Nutrition Research
Summary No. 4 / 2020
Belajová, E.
Optimization and applicability of high performance liquid chromatographic methods for quantification of patulin and ochratoxin A in fruit purées
Journal of Food and Nutrition Research, 59, 2020, No. 4, s. 290-300
Elena Belajová, Department of Chemistry and Food Analysis, Food Research Institute, National Agricultural and Food Centre, Priemyselná 4, P. O. Box 25, SK-82475 Bratislava, Slovakia.
Received 11 May 2020; 1st revised 20 July 2020; 2nd revised 6 October 2020; accepted
Summary: Patulin and ochratoxin A are mycotoxins generated in apples by certain strains of fungi Penicillium spp. and Aspergillus spp. Present work was aimed at occurrence of these mycotoxins in processed fruit-based and fruit-cereal mixed purées for infants and small children. For the purpose, two independent methods were optimized and verified. The methods were based on high performance liquid chromatography using diode-array detection and fluorescence detection and provided quantitative data on patulin and ochratoxin A contents, respectively. For sample pre-treatment, conventional liquid-liquid extraction was replaced with simpler procedures followed by solid phase extraction. The limit of detection was 5.8 µg·kg-1 for patulin and 0.06 µg·kg-1 for ochratoxin A, the limit of quantification was 6.9 µg·kg-1 and 0.12 µg·kg-1, respectively. The recoveries varied in the range of 79–92 % for patulin and 78–105 % for ochratoxin A at defined spiking levels. The described methods were applied in analyses of
Keywords: patulin; ochratoxin A; high performance liquid chromatography; fruit purée
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