Scientific journal

62 2023

Journal of Food and Nutrition Research
Summary No. 3 / 2023

Blaško, J. – Fulín, M. – Kubinec, R. – Duhačková, Ľ. – Kubincová, J. – Kukurová, K. – Blažková, M. – Kunštek, M. – Gábrišova, Ľ. – Kafková, V.
Fast differentiation of floral and honeydew honeys using gas chromatography-mass spectrometry
Journal of Food and Nutrition Research, 62, 2023, No. 3, s. 264-269

Róbert Kubinec, Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 84215 Bratislava, Slovakia. E-mail:

Original article
Received 21 May 2023; 1st revised 26 June 2023; accepted 27 June 2023; published online 11 August 2023

Summary: Distinguishing floral and honeydew honey using chemical analysis is generally problematic. This work solved this problem by determining the trisaccharide melezitose in honeys, which was identified as a potential marker for identification of the type of honey. The newly developed method for determination of melezitose reduces the analysis time by approximately 10 times and significantly simplifies sample preparation during derivatization of saccharides. A two-step procedure of silylation with hexamethyldisilazane followed by N,O-bis(trimethylsilyl)trifluoroacetamide was developed for derivatization without the formation of degradation products. The limit of detection of the method for melezitose was 4.9 mg·kg-1 based on the selected ion m/z of 361 with relative standard deviation for real samples of ≤ 15 %. The simplicity and speed of the method enables its use in screening tests. The method was successful at determination of a significant difference in the melezitose content of floral (103–2880 mg·kg-1) and honeydew honeys (10600–26100 mg·kg-1), which allowed differentiation between the types of honey.

Keywords: floral honey; honeydew honey; melezitose, rapid analysis; gas chromatography-mass spectrometry

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