Vedecký časopis - archív

46 2007

Journal of Food and Nutrition Research
Súhrny čísla 1 / 2007

Detection of Listeria monocytogenes in food in two days using enrichment and 5’-nuclease polymerase chain reaction with end-point fluorimetry
Journal of Food and Nutrition Research, 46, 2007, č. 1, s. 35-38

Tomáš Kuchta, Department of Microbiology and Molecular Biology, VÚP Food Research Institute, Priemyselná 4, P. O. Box 25, SK-824 75 Bratislava 26, Bratislava, Slovakia. E-mail:

Súhrn: A method for the detection of Listeria monocytogenes in food in two days is presented. The method consists of a two-step enrichment, bacterial cells lysis and a closed-tube duplex 5’-nuclease PCR with an internal amplification control, which is read in end-point mode in a 96-well fluorimeter. The 5’-nuclease PCR has an intrinsic detection limit of 104 and facilitates monitoring of false negative results caused by the failure of amplification. The complete method for the detection of L. monocytogenes in food has a detection limit of 100 CFU per 10 g and does not produce false positive results due to the presence of dead cells.

Kľúčové slová: Listeria monocytogenes; polymerase chain reaction; rapid method; end-point fluorimetry

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