Výskumný ústav potravinársky

Yoon, B. R. – Hong, S.-Y. – Cho, S. M. – Lee, K. R. – Kim, M. – Chung, S. H.
Aflatoxin M₁ levels in dairy products from South Korea determined by high performance liquid chromatography with fluorescence detection
Journal of Food and Nutrition Research, 55, 2016, č. 2, s. 171-180

Soo Hyun Chung, Sung-Yong Hong, School of Biosystem and Biomedical Science, Korea University, Anam-ro, Seoul 02841, South Korea. Tel: + 82-2-3290-5641; fax: +82-2-927-7825; e-mail: chungs59@korea.ac.kr (S. H. Chung); lunohong@korea.ac.kr (S.-Y. Hong)

Received 3 February 2016; 1st revised 17 March 2016; accepted 11 April 2016; published online 21 May 2016.

Súhrn: Sensitive methods for determination of aflatoxin M₁ (AFM₁) in milk, yoghurt and cheese were developed and evaluated. The analytical methods use high performance liquid chromatography-fluorescence detection after immuno­affinity column purification. The methods showed high linearity (r² ≥ 0.999) for AFM₁ in the range of 0.05–10.00 µg·l^–1. The limit of quantification for AFM₁ was 0.003 µg·kg^-1, 0.07 µg·kg^-1 and 0.05 µg·kg^-1 in milk, yoghurt and cheese, respectively. The recovery rate of AFM₁ in the dairy products was 83–108% along with 2.1–12.8% repeatability and 0.0–13.1% reproducibility. Levels of AFM₁ were determined in 224 samples of milk, yoghurt and cheese collected from South Korea. The dairy samples were contaminated with AFM₁ in the range of 0.001–0.100 µg·kg^-1 for milk and 0.015–0.136 µg·kg^-1 for yoghurt and cheese. The levels of AFM₁ in the dairy products did not exceed the maximum limit (0.5 µg·kg^-1) set for milk by Korea Food and Drug Administration.

Kľúčové slová: aflatoxin M₁; milk; yoghurt; cheese; high performance liquid chromatography; fluorescence detection

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